Formation of empty B19 parvovirus capsids by the truncated minor capsid protein
نویسندگان
چکیده
منابع مشابه
Parvovirus B19 empty capsids as antigen carriers for presentation of antigenic determinants of dengue 2 virus.
For the production of dengue-vaccine candidates, empty capsids, or virus-like particles (VLPs), of parvovirus B19 that carry dengue 2-specific epitopes were employed as antigen carriers. Two epitopes (comprising amino acids 352-368 and 386-397) of domain BIII of the envelope glycoprotein were chosen to produce recombinant B19 VLPs for immunization of BALB/c mice. Serum samples from immunized mi...
متن کاملCryo-electron microscopy studies of empty capsids of human parvovirus B19 complexed with its cellular receptor.
The three-dimensional structures of human parvovirus B19 VP2 capsids, alone and complexed with its cellular receptor, globoside, have been determined to 26 resolution. The B19 capsid structure, reconstructed from cryo-electron micrographs of vitrified specimens, has depressions on the icosahedral 2-fold and 3-fold axes, as well as a canyon-like region around the 5-fold axes. Similar results had...
متن کاملA genetically engineered cell line that produces empty capsids of B19 (human) parvovirus.
B19 parvovirus is pathogenic in humans, causing the common childhood exanthem fifth disease and bone-marrow failure, both acute (transient aplastic crisis of hemolysis) and chronic (pure erythrocyte aplasia in immunodeficiency). The virus is tropic for a human red cell progenitor cell, and failure to culture B19 in a cell line has limited its clinical study. We cotransfected the right half of t...
متن کاملHuman parvovirus B19 VP2 empty capsids bind to human villous trophoblast cells in vitro via the globoside receptor.
BACKGROUND Pregnant women acutely infected with human parvovirus B19 (B19) may transmit the virus to the developing fetus. The mechanism whereby the virus interacts with the placenta is unknown. It is known that globoside receptor is required for successful infection of the target cells, which are the highly undifferentiated, actively dividing colony and burst-form units of the erythroid series...
متن کاملDetermination and refinement of the canine parvovirus empty-capsid structure.
The canine parvovirus (CPV) empty-capsid structure has been determined and refined to 3.0 A resolution in the tetragonal space group P4(3)2(1)2 with cell dimensions a = b = 254.5 and c = 795.0 A. The successful structure determination shows that reasonably good diffraction data were obtained in spite of the very long c axis. The structure was solved by molecular replacement using the electron d...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1994
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.68.7.4690-4694.1994